DNA

Part:BBa_K2100073:Design

Designed by: Colleen Foley   Group: iGEM16_MIT   (2016-10-19)


pEXPR EGSH - 2kturn: mKate


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1287
    Illegal XbaI site found at 2193
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1287
    Illegal NheI site found at 832
    Illegal NheI site found at 1098
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
    Illegal NotI site found at 2207
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1287
    Illegal BglII site found at 1410
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1287
    Illegal XbaI site found at 2193
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1287
    Illegal XbaI site found at 2193
    Illegal PstI site found at 1780
    Illegal PstI site found at 2200
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4042
    Illegal SapI.rc site found at 709
    Illegal SapI.rc site found at 3424


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.

Source

We synthesized this part using LR Gateway cloning.

References